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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene is noticed in several tumors, which results in chromosome instability, and thus may possibly add to tumorigenesis. This protein was discovered to affiliate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a vital purpose in advertising cell-cycle progression while in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, are actually explained. Two supplemental splice variants were being reported but comprehensive nucleotide sequence info just isn't yet accessible.

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Blocking peptides are peptides that bind exclusively towards the concentrate on antibody and block antibody binding. These peptide typically contains the epitope identified from the antibody.

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This information outlines additional feasible optimization of mobile and immunoassay parameters to be sure the absolute best success are...

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This guidebook outlines further probable optimization of cellular and immunoassay parameters to make sure the very best final results are...

Antibodies sure to the blocking peptide no longer bind towards the epitope over the target protein. By evaluating the staining within the blocked antibody compared to the antibody by yourself, you can see which staining is particular.

Focus on Information and facts Cyclin E belongs for the remarkably conserved cyclin loved ones, whose associates are characterised by a spectacular periodicity in protein abundance through the cell cycle. Cyclins operate as regulators of CDK kinases. Distinctive cyclins exhibit distinctive expression and degradation styles which contribute towards the temporal coordination more info of each mitotic celebration. Cyclin E varieties a posh with and capabilities as being a regulatory subunit of CDK2, whose activity is required for mobile cycle G1/S changeover. This protein accumulates at the G1-S phase boundary and is degraded as cells development through S section.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by each antibodies...

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